International Journal of Applied Science and Technology

ISSN 2221-0997 (Print), 2221-1004 (Online) 10.30845/ijast

Comparative Examination of Deep-Frozen Ram Semen after Thawing and Incubating an Different Solution
A. Csiba, E. Gyoker, E. Gergatz, N. Gombkoto

Optimizing the cryopreservation technology of ram semen is a very important task. In our institution we try to improve the different stages of the cryopreservation procedure- the preparing (dilution and precooling), deepfreezing and thawing. In the literature review we compared the own preparing and deep-freezing methods with different methods, that were used by another authors. In our study we examined the quantity of ejaculates in spring and autumn, the changes of motility% and the capacitation status of ram semen samples after the thawing and incubation (39ºC, 2h) in different thawing and incubation solutions. The thawing and incubation solutions contain PBS (phosphate buffer solution) without- (control solutions) and with different addition materials (semen plasma, different ram’s and ewe’s blood serum). In our experiments we determined the changing of the motility%, the capacitation status and the rate of acrosome-reacted cells after the thawing and incubation (at 39ºC, 2h).

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