Determination of Copper (II) in Fungal Drugs, Vehicle Exhaust Particulates and Water Samples with a Novel Kinetic Spectrophotometric Method Based on Its Inhibitory Effect on Redox Reaction between Giemsa Stain and Ascorbic Acid
Esra Bagda

Abstract
A new kinetic method is described for determination of trace levels of Cu (II) based on its inhibitory effect on the redox reaction between l-ascorbic acid (LAA) and giemsa stain. The decolorization of giemsa stain by the LAA was used to monitor the reaction spectrophotometrically at 662 nm. The fixed time method used for different time intervals from the initiation of the reaction. The method allows the determination of Cu (II) in the range of 0.1-8.0 μg ml-1 ( r2=0.9911) for 0.5-5 min. The relative standard deviation was 2.8 % for 10 determination of 2.0 μg ml-1 of Cu (II) and the limit of detection (3Sb/m) was 0.188 μg ml-1. The proposed method was applied successfully to determination of Cu (II) in fungal preparation, tap water and NIES CRM No: 8 vehicle exhaust particulates. The results of the method were satisfactory compared to with AAS results.

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