Arsenic-Sensitivity in Anadenanthera Peregrina Due to Arsenic-Induced Lipid Peroxidation
Marcelo Pedrosa Gomes; Marília Carvalho, Teresa Cristina Lara Lanza Sá e Melo Marques; Daniela Moreira Duarte; Claudia de Oliveira Gonçalves Nogueira; Ângela Maria Soares; Queila de Souza Garcia

Abstract
Anadenanthera peregrina is legume that can grow on arsenic-contaminated sites when colonized by arbuscular mycorrhiza fungi (AMF). Previous studies demonstrated that these plants are subject to arsenic-toxicity in the absence of AMF. The present study investigated As-sensitivity in A. peregrina by submitting plants to increasing As concentrations (0, 10, 50 and 100 mg kg-1) while monitoring alterations in their growth, root anatomical and morphology and associated biochemical features (oxidative damage and antioxidant enzymes). Our results demonstrated that arsenic affects early root growth, inducing root anatomical changes and altering plant antioxidant scavenging machinery by inactivating some antioxidant enzymes (CAT and APX). As-induced oxidative stress caused by lipid peroxidation (but not by H2O2 accumulation) may be the main cause of As-sensitivity. Since these plants become As-tolerant under AMF symbioses, the ability of AMF to minimize oxidative stress under these conditions needs to be more closely investigated.

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