Investigating the Cellular Response of Copper (II) Ions on both Cancerous and Noncancerous Cell Lines: A Closer Look into MCF-7, A375, and HFF Cells
Amy J. Heston, Ph. D; Michelle L. Colopy, Christine N. Stenger, Lucielle E. Zappitelli

Abstract
Copper compounds are known for their toxicity toward bacteria and a limited number of viruses. This project investigated the effects of copper compounds on breast cancer, skin cancer and noncancerous cell lines. These metal ions may enter the cell through the Na+/K+ pump, disturb chemical processes, and, therefore, could lead to cell death. These cell lines were treated with various concentrations of CuCl2•2H2O. A Sulforhodamine B assay monitored overall cell death. The antiproliferative effects of the cancerous cells were compared to that for the noncancerous cells and allowed for unique projects for two undergraduate nursing majors and one honors nursing major. This work enhanced the students’ understanding and critical thinking in inorganic chemistry and its real-life applications. Interestingly, the data support that Copper (II) ions possess anticancer properties and have the ability to kill cancer cells, in vitro.

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