THE BREAST OF ANTICANCER FROM LEAF EXTRACT OF ANNONA MURICATA AGAINTS CELL LINE IN T47D
Eka Prasasti Nur Rachmani, Tuti Sri Suhesti, Retno Widiastuti, Aditiyono

Abstract
The breast cancer is the second leading cause of death in women after cervical cancer. The soursop (Annona muricata) is a traditional medicinal plant which is empirically by the people of Indonesia are used for anti-inflammatory and anti-tumor. This study aims to determine the cytotoxic effects from extracts of leaves of soursop and fraction results in cancer cells T47D. The research was carried out by extraction using ethanol and fractionation by column chromatography method that used various solvents were n-hexane, chloroform, ethyl acetate and methanol. Cytotoxic test performed by the method of MTT assay and apoptosis tests performed by the method of Double Stainning. The parameters obtained from the cytotoxic test was IC50 values, ie values that produce inhibitory concentrations of cancer cells by 50%. Apoptosis assay results are analyzed in a qualitative description. The results showed that the ethanol extracts of leaves of the soursop has a cytotoxic activity with IC50 values of 17.149 μg / mL. The results of the four fractions obtained by fractionation and the fraction F3 were the fraction that has the best cytotoxic activity with IC50 values of 30.112 μg / mL. Apoptosis assay results showed that the fraction F3 were able to induce apoptosis of cells.

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