RAPD Analysis of Stripe Rust Resistant Synthetic Hexaploid of Wheat
Dr Sumaira Farrakh, Dr Iftikhar Ahmad, Dr Javed Iqbal Mirza, Dr. Shahid Hameed, Dr Mujeeb Kazi, Dr Muhammad Ashraf

Abstract
In this study 33 lines of synthetic hexaploid (SH) of wheat were used for RAPD analysis. These 33 SH were found resistant for stripe rust during the two years of screening at seedling and adult plant stage in field and glasshouse. Total 50 primers were screened and 10 primers of OPA series which were giving the reproducible band were selected for the study of genetic diversity. The result showed the stripe rust resistant synthetic hexaploid of wheat are highly diverse. A total of 108 bands were generated by the ten selected primers, 88 of which were polymorphic. Maximum level of polymorphism (93%) which was observed for the primers OPA-01 while minimum level of polymorphism (36%) was observed by OPA-19 primers. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 33 lines into two groups. Eight lines did not form cluster with any of the genetypes and found to be very diverse. These lines can be used by the plant breeders of the country to improve the stripe rust status of the susceptible wheat varieties.

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