Influence of Age, Sex and Breed Lines of Zebu Nellore Cattle on the Erythrocytes Osmotic Fragility, raised in São Paulo, Brazil
Maria Consuêlo Caribé Ayres, Eduardo Harry Birgel, Eduardo Harry Birgel Júnior, Laura Emilia Panelli Martins

Abstract
The average standard values of erythrocytes osmotic fragility (EOF) were established to zebu cattle of Nellore, raised in State of São Paulo , by analyzing the influence of age, sex and breed lines (Standard Nelore, Lemgruber Nellore, Mocho Nellore and Kuleia Nellore). For this purpose, 230 samples of blood from healthy animals free from Leucosis, Brucellosis, and blood parasites, were collected and submitted to standard hematological techniques and the EOF test. Blood samples obtained from calves up to three months showed the lesser fragility to hypotonic saline (50% of hemolysis= 0,43±0,03g/dL NaCl). Groups older than three months to over 72 months (50%= 0,58±0,03 to 0,48±0,02 g/dL NaCl) showed EOF decrease. The EOF was statistically different between the varieties Mocho (50%=0,54±0,05 g/dL NaCl) and Kuleia (50%=0,48±0,06g/dL NaCl). Sex had no influence. The average standard values of the EOF in 50% of hemolysis to Zebu Nellore cattle were 0,51±0,04g/dL NaCl.

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