Lipase and Surfactant Production by Fungi Isolated of Oily Residues of Environmental Sanitation in Liquid and Solid Phase Reactors
Celson Rodrigues, Sérvio Túlio Alves Cassini, Paulo Wagner Pereira Antunes, Laura Marina Pinotti, Regina de Pinho Keller, Ricardo Franci Gonçalves

Abstract
The use of lipolytic activity of fungal biomass as biocatalyst represent an attractive approach for oily waste treatment and or production of biodiesel from oils and greases wastes. Fifteen isolates of filamentous fungi and five of yeasts from oily residues were obtained and our lipolytic activity and biomass production were quantified with 120 h of growth in a minimal mineral media with 10 % of soybean oil. Lipolytic activity varied from 0.13 U.mg-1 protein for Rhizomucor sp. F18 to 18.06U.mg-1protein for Penicilliumsp F2 and the biomass varied from 7.61 mg.mL-1 for Cladosporium sp. F28 to 12.68 mg.mL-1 for Rhizomucor sp. F18. Penicilliumsp F2 and Rhizomucor sp. F18were then evaluated in liquid phase reactor and solid phase reactor for lipolytic activity, surfactant activity, biomass production and removal of oils and greases of substrate, and qualified to future tests as whole-cell biocatalysts for hydrolysis and transesterification processes.

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